Note.—If only microscopical films are needed, fill up the centrifuge tube with Ligroin (a petroleum ether) in place of sterile distilled water in step 5 and prepare the films from the surface of the fluid, to stain by the Ziehl-Neelsen process.
The Elements of Bacteriological Technique / A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
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About This Book
A practical laboratory guide presents concise, step-by-step methods for bacteriological work aimed at medical, dental, and technical students. It explains laboratory organization, glassware selection and cleaning, plugging, sterilization procedures and timing devices. Microscopy, staining techniques, tissue section methods, and the classification and basic physiology of moulds and bacteria are described alongside biochemical tests. The book gives detailed instructions for preparing and standardizing nutrient and special media, incubators, aerobic and anaerobic cultivation, isolation and identification procedures. It covers experimental inoculation of animals, observations during infection, post-mortem methods and serological assays such as agglutination and opsonisation. Practical bacteriological analyses of water, milk and dairy products are included, supported by numerous illustrations and apparatus diagrams.