The preparation & mounting of microscopic objects

INDEX.

• Air-bubbles, 56.
• Air-pump, 13;
• use of, 57.
• Alcock, Dr., on tongues of Mollusca, 118.
• Algæ, mounting of, 91.
• Anacharis alsinastrum, rotation in, 143;
• to cultivate, 144.
• Animal tissues, dissection of, 114.
• Antennæ of insects, 71;
• in preservative liquid, 94.
• Apparatus required in mounting objects, i.
• Arteries, how to distinguish, in injection, 131.
• Asparagus, spiral vessels of, 114.
• Asphaltum, 15;
• and india-rubber, as cement and varnish, 18.
• Bat, circulation of blood in wings of, 142.
• Beale’s, Dr., Prussian blue for injecting, 134;
• carmine, 135.
• Bell-glasses, use of, 12.
• Berg-mehl, 40.
• Bermuda earth, 40.
• Bichromate of lead, injection with, 132.
• Bird, Dr. Golding, on preparation of Zoophytes, 65.
• Black-japan, as a cement, 16.
• Black varnish, 18.
• Blood, as a microscopic object, 50;
• circulation of, 140–142.
• Bone, sections of, 102;
• fossil, 103.
• Brass plate for heating glasses, &c., 13.
• Cactaceæ, raphides of, 52.
• Camel-hair pencils, use of, 10.
• Canada balsam, 14;
• with chloroform, 14, 60, 132;
• with turpentine, 14;
• air bubbles in, 56;
• Dr. Carpenter’s syringe for, 59;
• to fill cells with, 60;
• mounting of objects in, 56.
• Carbolic acid, 86.
• Carmine injecting fluid, Dr. Beale’s, 135:
• Dale & Davies’, 138.
• Cartilage, dissection of, 114.
• Castor oil, as a preservative, 86.
• Cells for dry objects, 6;
• with rings of cardboard, 7;
• with gutta-percha, 7;
• with leather, 7;
• with ivory, 8;
• with thin glass, 7;
• with varnish, 23, 26;
• for balsam, 60;
• for preservative liquids, 87–89;
• Shadbolt’s turntable useful in making, 9.
• Cements, 13–17.
• Chalk, foraminifera from, 63.
• Chara vulgaris, rotation in, 144.
• Chloride of zinc, solution of, 85;
• of calcium, solution of, 92.
• Chloroform, use of (see Canada balsam).
• Circulation of blood, 140–142;
• of sap (see Rotation).
• Cleanliness in microscopic work, 1.
• Coal, sections of, 99.
• Collection of diatoms, 29.
• Colours for injection, 125–127.
• Condenser, cheap, to make, 20.
• Corals, sections of, 99.
• Corallines, to mount, 48.
• Cover of objects, to remove, 60.
• Crystals, mounting of, dry, 50;
• to vary form of, and mount in balsam, 75;
• sections of, 109.
• Cuticle of equisetum, &c., 80.
• Dale & Davies’, carmine injection, 138.
• Deane’s gelatine, 84.
• Decalcifying process for shells, 98.
• Desmidiaceæ, in preservative liquid, 92.
• Diachæa elegans, 53.
• Diamond beetle, 54.
• Diatomaceæ, nature of, 28;
• collection of, 29;
• in stomachs of fish, 30;
• to prepare and mount dry, 30–40;
• Mr. Rylands on, 33;
• in guano, 39;
• fossilized, 40;
• mounting in balsam, 61;
• mounting in preservative liquids, 92, 93.
• Discs used in mounting, 22;
• supporter, Smith & Beck’s, 23.
• Dissection, 111;
• microscope for, 111;
• instruments for, 112.
• Distilled water, as preservative liquid, 83.
• Doyer’s, M., method of injection, 132.
• Dry objects, to mount, 22.
• Echinodermata spines, sections of, 99.
• Eel, scales of, 78.
• Eggs of insects, 94.
• Elastic objects, to keep flat upon the slide, 11.
• Electrical cement, 16.
• Equisetaceæ, 80;
• spores of, 146.
• Erector, not indispensable, 19.
• Eyes of insects, 70, 54;
• of animals injected, 136.
• Feathers, 73.
• Feet of insects, 54, 71.
• Ferns, 51;
• development of spores of, 145.
• Fish, fins and tails of, 50;
• scales of, 53, 78;
• injection of, 137;
• circulation of blood in, 142.
• Flint, sections of, 101.
• Fluid, mounting objects in, 83.
• Flustra avicularis, 79.
• Fly (see Insects).
• Foot of sheep injected, 136.
• Foraminifera, 41;
• separation of, 41;
• to clean, 42;
• to clean from tallow soundings, 42;
• to mount, 45, 63;
• from chalk, 63.
• Forceps, ordinary and wooden, 11;
• bull-nosed, for injection, 123.
• Fossil infusoria, 40.
• Frog, injected, 136;
• to show circulation of blood, 140.
• Frog-bit, rotation in, 144.
• Fruit-stones, sections of, 104.
• Fungi, 53, 94.
• Gastric teeth of insects, 120;
• of molluscs, 117.
• Gelatine for injection, 124;
• preservative liquid, 84.
• Glass slides, 1;
• thin, 3;
• thin, to cut, 3;
• thin, to measure, 4;
• thin, to clean, 5;
• cells, to make, 88;
• rings for cells, 88;
• tubes, 10.
• Glycerine, 84, 94;
• jelly, 84, 91.
• Goadby’s fluid, 85.
• Gold-size, 15.
• Grasses, 80.
• Grasshopper, gizzard of, 120.
• Guano, containing Diatomaceæ, 39.
• Gum-water, and modifications of, 17.
• Gutta-percha cells, 7;
• for liquids, 87.
• Hairs, vegetable, 46;
• to mount dry, 54;
• to mount as polarizing objects, 79;
• sections of, 105.
• Hepworth, Mr., on mounting insects, 68.
• Horn, sections of, 104.
• Hot-water bath, use of, 58.
• Infusoria, in preservative liquid, 92;
• fossil, 40.
• Injections, vi., 122;
• apparatus for, 122;
• colours for, 125;
• directions for, 127;
• with various colours, 131;
• mounting of, 131;
• transparent, 133, 138.
• Insects, scales of, 48;
• to mount, 49;
• legs and feet of, 54, 72;
• eyes of, 54, 70;
• Mr. Hepworth on mounting, 68;
• antennæ of, 71;
• mouth of, 72;
• tracheæ and spiracles of, 72, 115;
• parasitic, 73;
• in preservative liquid, 94;
• eggs of, 94;
• gizzard of, 120;
• circulation of blood in, 142.
• Intestines, injected, 136.
• Knives for dissecting, 112;
• Valentin’s, 108.
• Labelling of objects, 19.
• Lamps, for mounting, 12.
• Larvæ, skins of, 50.
• Leaves, sections of, 107;
• scales of, 46, 81.
• Liquid-glue, 16.
• Lungs of animals injected, 137.
• Mallow, pollen of, 47.
• Marine glue, 15, 88.
• Microscope for dissection, 111.
• Miscellaneous, vii., 140.
• Mites, 73.
• Molluscs, tongues of, 116.
• Mosses, 51;
• in preservative fluids, 91.
• Mould (see Fungi).
• Mounting objects, apparatus for, i.;
• dry, ii., 22;
• in Canada balsam, iii., 56;
• in cells, iv., 83.
• Mouse, ear of, 114;
• circulation of blood in, 142.
• Mouth of insects, 72.
• Muscle, dissection of, 114.
• Needles, how to mount, 10;
• for dissection, 112;
• curved, for injection, 123.
• Nervous tissue, dissection of, 115.
• Nettle leaf, 52.
• Newts, injected, 136.
• Onion, raphides of, 52.
• Orbitolite, section of, 97.
• Oxalurate of ammonia, crystals of, 75.
• Palates of Molluscs (see tongues).
• Papers, ornamental, to cover slides, 8, 27.
• Photographs, microscopic, to produce, 147;
• Mr. Shadbolt on, 149.
• Pipes for injecting syringe, 122.
• Podura, scales of, 49.
• Polariscope, objects for, 74–82, 104.
• Pollen, 47, 74.
• Polycystina, preparation and mounting of, 63.
• Preservative liquids, iv., 83;
• cells suited for, 87–89.
• Prussian blue for injection, 134.
• Raphides, vegetable, 52, 80.
• Rhinoceros, horn of, 104.
• Rhubarb, spiral vessels of, 113.
• Rings and cross of crystals, 109.
• Rotation of fluid in cells of plants, 143–145.
• Rush, section of, 108.
• Rylands, Mr. T. G., on Diatomaceæ, 33, 93.
• Salicine, crystals of, 76.
• Saw of watch-spring, 97.
• Scales of fishes, 53, 78;
• of leaves, 46, 81;
• of insects, 48.
• Scissors, 10;
• for dissection, 112.
• Sea-mats, 53.
• Sea-soundings, to cleanse, 42.
• Sealing-wax varnish, 17.
• Sections, 96;
• of shells, 97;
• of orbitolite, 97;
• of spines of Echinodermata, 99;
• of corals, 99;
• of coal, 99;
• of flint, 101;
• of teeth, 101;
• of bone, 102;
• of fruit-stones, 104;
• of horn, 104;
• of whalebone, 105;
• of hairs, 105;
• of wood, 106;
• of leaves, 107;
• of sponges, 108;
• of skin, 109;
• of crystals, 109;
• of seeds, 111.
• Seeds, 47, 74;
• sections of, 111;
• growth of, 145.
• Shadbolt’s turntable, 9.
• Shells, sections of, 97;
• decalcifying, 98;
• laminæ of, 98.
• Siliceous cuticles, 80.
• Size for injection, 124.
• Skins of larvæ, 50;
• sections of, 109;
• sole, 54.
• Slides, glass, for mounting objects, 1;
• glass, to clean, 2;
• wood, &c., 6;
• most useful, 8;
• to cover and varnish, 27.
• Spicula, from sponges, &c., 67.
• Spines of Echinus, 99.
• Spiracles of insects, 72, 116.
• Spiral vessels of vegetables, 113.
• Split bristles, use of, 10.
• Sponges, sections of, 108.
• Spores of ferns, development of, 145;
• equisetum, 146.
• Starch, preparation and mounting of, 79.
• Sulphate of copper and magnesia, crystals of, 76.
• Syringe for Canada balsam, 59;
• for dissection, 113;
• for injection, 122.
• Tadpole, to show circulation of blood of, 141.
• Teeth, sections of, 101.
• Thin glass, to cut, 3;
• to measure thickness of, 4;
• to clean, 5.
• Thwaites’ preservative liquid, 85.
• Ticks, 73.
• Tissues, animal and vegetable (see Dissection).
• Tongues or palates of Molluscs, 116.
• Tracheæ of insects, 72, 115.
• Transfer of objects, 10.
• Trough for dissection, 113.
• Tubes, glass, 10.
• Turnbull’s, Dr., Prussian blue for injection, 134.
• Turpentine, use of, 57.
• Universal stand, to make, 20.
• Valentin’s knife, 108.
• Vallisneria spiralis, rotation in, 143;
• to cultivate, 144.
• Varnishes, 17.
• Vegetable objects, to mount dry, 46;
• to mount in jelly, 91;
• dissection of, 113.
• Watch-glasses, 12.
• Whalebone, sections of, 105.
• Wood, sections of, 106.
• Zoophytes, to mount dry, 53;
• Dr. Golding Bird on mounting, in balsam, 65;
• as polarizing objects, 79.