Glenodinium cinctum Ehr. Fig. 22.

The body is globular, smooth, and homogeneous. Brown chromatophores arranged radially, each in the form of a cone, the base of which rests against the shell while the points turn inward. A bright-red eye-spot may or may not be present; when present it is placed near the junction of the two furrows. The longitudinal furrow is small. Fresh water and salt.

Length and diameter the same, 21µ.

This species was observed by Peck '93.

[Illustration: Fig. 22.—Glenodinium cinctum.]

Genus PERIDINIUM Ehr. '32, Stein '83.

The form is globular, ovoid or elongate, the apex frequently drawn out into a long tube. The transverse and longitudinal furrows are quite distinct, the former having often a spiral course about the body. The two halves of the body are similar, the posterior being somewhat shorter; the anterior half has seven equatorial plates, an oral plate, two lateral apical plates, and one or two dorsal plates. The two antapical plates frequently have a tooth-like process. The bodies are colorless, green or brown.

Fresh and salt water.

Peridinium digitale Pouchet. Fig. 23.

Synonyms: Protoperidinium digitale Pouchet; Protoperidinium Bergh p. p.; P. divergens Peck.

The shell is covered with pits of large size. The posterior part is hemispherical and surmounted by a single horn or spine. The transverse furrow is very oblique, and its two extremities are united by a sigmoid longitudinal furrow. The anterior half bears two spines or horns of different size, and variable. The nucleus is spherical or ellipsoidal and placed in the posterior half of the shell.

Length 68µ; diameter 54µ. Common.

Although the description of Pouchet's P. digitale differs in some respects from a careful description of the Woods Hole form, I think the species are the same. The chief difference is in the single horn of the posterior half; in Pouchet's form this is furrowed by a narrow groove which runs to the S-shaped longitudinal furrow. In the Woods Hole form I was unable to make out such a furrow. The flagella, also, were not seen. This same form was pictured by Peck '95 as P. divergens.

Peridinium divergens Ehr. Fig. 24.

Synonym: Ceratium divergens Kent.

The shell is spheroidal, widest centrally, attenuate and pointed posteriorly; the anterior portion is armed with two short, pointed horns, each of them having a toothed process at the basal portion of the inner margin. They are frequently colorless and beautifully transparent, the body being free from large opaque granules; again they are colored brown or yellow. The nucleus is large and elongate and finely granular. 75µ long and 68µ in diameter. Common.

Genus CERATIUM (Schrank).

The general shape is a flattened sphere with three long processes or horns. The cross-furrow is either spiral or circular; the longitudinal furrow is usually wide and occupies the greater part of the anterior half of the shell. The shell is thick, reticulate or striped, and sometimes provided with short spines; often distinctly porous. The anterior half is composed of 3 equatorial and 3 apical plates, the latter being continued into the horn-like process. The posterior half is composed of 3 equatorial and one apical plate continued into the posterior horn. The right posterior plate is continued into a similar horn which may remain rudimentary or be continued into a considerable process. Similarly the left posterior horn is usually developed, but remains small. There may be from 2 to 3, 4, and 5 horns. Chromatophores usually present, green to yellow brown.

Fresh and salt water.

Ceratium tripos Ehr. Fig. 25.

The body is somewhat triangular and bears three horns, two of which are shorter than the other one and slightly curved upward.

Length, including the horns, 290µ.

Ceratium fusus Ehr. Fig. 26.

Synonym: Peridinium fusus Ehr.

The animal is very elongate, due to the presence of two long horns at the extremities of the body. Color, yellow with chromatophores. Length 285µ; width 23µ.

Both of these species are common in the tow and in the algæ at the edge of the wharf. Both of them are mentioned by Peck in '93 and '95.

Genus AMPHIDINIUM Clap. & Lach.

The body is ovoid to globular and usually much flattened dorso-ventrally. The anterior portion is very much reduced and is somewhat head-like or cap-like. The longitudinal furrow extends through the entire posterior body length and is apparently capable of widening and narrowing. It is probably naked (see here Klebs, Pouchet, Bütschli), although Stein maintained that there is a delicate cuticle-like shell. Chromatophores of brown or green colors present and usually grouped radially about a central amylum granule. The nucleus is posterior.

Fresh and salt water.

Amphidinium operculatum Clap. & Lach. Fig. 27.

The body is oval and flattened. The transverse furrow is at the extremity (posterior) of the body and the small portion, which is thus apparently cut off, is the cap-like or operculum-like structure which gives the name to the species. Klebs maintains that the two furrows are not connected, but in this he is certainly mistaken, provided we have the same species under consideration. Very common about Woods Hole.

Length from 40 to 50µ; width 30µ; thickness 15µ.

Genus LACRYMARIA Ehr. '30.

Body short to very long flask-shape; for the most part contractile, especially in the neck region. The posterior end is rounded or pointed. The main character is the mouth-bearing apex, which "sets like a cork in the neck of the flask." One or more circles of long cilia at the base of the mouth portion or upon it. The body is spirally striped. Contractile vacuole terminal, with sometimes one or two further forward. Macronucleus central, globular to elongate, sometimes double. Food mainly bacteria. Fresh and salt water.

Lacrymaria lagenula Clap. & Lach. Fig. 28, a, b.

Synonym: L. tenuicula Fromentel '74.

Body more or less flask-shape, two or three times as long as broad, with conical apex, which is slightly elastic and protrusible; surface obliquely striate, with well-defined lines, 14 to 16 in number; cilia uniform on the body, with a crown of longer ones at the base of the conical proboscis. The body cilia are not thickly placed except around the proboscis. The endoplasm is thickly packed with large granules (food particles) in the anterior half and with finely granular particles in the posterior half. The elongate macronucleus lies a little above the center among the larger granules; the contractile vacuole is double, one on each side of the median line and at the posterior end of the body among the finer granules. The anus is posterior. Length 90µ to 160µ; greatest width assumed 65µ. When fully expanded the posterior end assumes a curious polyhedral form. (Fig. 28 b.)

This form differs slightly from others of the same species as described by different observers, the most striking difference being the presence of two contractile vacuoles in place of the usual one. These are very slow to fill and grow to a large size before diastole. The membrane is very tough and retains its form easily under pressure of the cover glass. Another characteristic feature is the flattening of the surfaces between the striæ. Decaying algæ.

Lacrymaria coronata Cl. & Lach. '58. Fig. 29.

Synonyms: L. lagenula Cohn '66; Möbius '88; L. cohnii ? Kent '81; L. versatilis Quen. '67.

Form flask-like and similar to L. lagenula, contractile but tough. The contractile vacuole is terminal, the proboscis is short, slightly raised and separated from the body by a deep cleft; the buccal cilia are inserted part way up on the proboscis. Form changeable, from short, sac-like to elongate and vermiform. Length 85µ.

This species is not very different from L. lagenula, but I noted that in addition to the elongate nucleus, the body striæ are much more apparent here and seem to sink into the cuticle, giving the periphery, especially at the collar region, a curious crenulated effect. The endoplasm is very densely granular and colored a blue-green, probably from food particles. The number of striæ is much larger than in the preceding species. The membrane is very tough and retains the shape of the body, even with the full pressure of the cover glass. Micronucleus and trichocysts were not observed.

Genus TRACHELOCERCA (Ehr. '83) Cohn '66.

The only well-known representative is very elongate, large (up to 3 mm. Van Beneden), and very contractile. The main feature of importance in distinguishing it is the 4-part structure of the mouth region, which, however, may not be obvious. Pharynx faint and smooth. Contractile vacuole terminal. Macronucleus in one central body or in numerous pieces scattered throughout the cell. Salt water.

Trachelocerca phœnicopterus Cohn '66. Fig. 30.

Synonyms: T. sagitta Ehr. '40, Stein '59; T. tenuicollis Quennerstedt '67, Kent '81; T. minor Gruber '87, Shevyakov '96.

The body is extremely elongate and ribbon-like, and this, combined with its wonderful power of extension and retraction, makes it one of the most curious and interesting of microscopic forms. The anterior end is square or cylindrical; the type species has a four-sided mouth, but many specimens may be found which have a plain cylindrical mouth region. One reason for this may be the fact that the extremity gets broken off. In one instance I noticed a very large form with the anterior end under some debris, which evidently held it tight, for the body of the ciliate was thrashing back and forth and twisting itself into knots, etc., like a nematode worm. Finally, the anterior end broke off with about one-tenth of the body; the remainder, in an hour, had regenerated a new anterior end with long cilia, but with no indication of four sides. The small anterior piece was also very lively, moving about and eating like the normal animal; its history, however; was not followed. This species appears to be variable in other ways as well; thus, in some cases the posterior end is rounded (cf. Entz '84); in others it is pointed (cf. Kent '81, Cohn '66, et al.).

Again, the macronucleus may be a single round body (Entz '84, Bütschli '88) or in two parts (Kent '81), or in many parts scattered about the body (Gruber). In the Woods Hole forms the tail is distinctly pointed and turned back sharply, forming an angle at the extremity. The cilia on this angular part are distinctly longer than the rest. The function of this posterior part is apparently to anchor the animal while it darts here and there upon the tail as a pivot, contracting and expanding the while. The body is finely striated with longitudinal markings; when contracted there are no transverse markings nor annulations. The nucleus is in the form of many fragments scattered throughout. Length of large specimen 1.7 mm.

Genus MESODINIUM Stein '62.

The main part of the body is globular or conical, with a short, platform-like oral region, and a deep annular groove about the middle of the body. The œsophagus is rather long, and smooth or longitudinally striped. One or more rings of cirri rise in the groove. If more than one ring of cirri are present, the anterior set usually point forward and lie close to the anterior part of the body. The posterior set, on the other hand, cling close to the posterior region of the body and give to it a peculiar encapsuled appearance. The most characteristic feature is the presence of four short tentacle-like processes which can be protracted and retracted from the oral region. (Mereschowsky says that the entire anterior half is more or less contractile.) The macronucleus is horseshoe-shaped or ovoid and is situated in the posterior half of the body. The contractile vacuole is also posterior.

Movement consists in rapid swimming, with rotation on its axis, or in creeping by means of its anterior cirri, or in sudden jumping, by which it apparently clears a distance of 20 times its diameter in one bound. Mouth parts may also be used for attachment to foreign bodies. The moving periods alternate with quiescent periods, during which the organisms with their outstretched and radiating cirri resemble the heliozoön Actinophrys.

Mesodinium cinctum, n. sp. Fig. 31.

Body spherical to pyriform, constricted near the middle, the constriction dividing the body into dissimilar parts. The anterior part is broadly pyriform, somewhat plastic and hyaline, with an oral extremity which is sometimes hollow, sometimes evaginated and convex. Upon this flexible anterior part there are four short but distensible tentacles. The posterior part is granular and usually filled with food particles; it is well rounded and holds the nucleus and contractile vacuole. The entire body is surrounded by a fine cuticle. The nucleus is elongate and extends through the greater part of the posterior half. The contractile vacuole lies on one side, near the girdle. The mouth is on the anterior pole in the tentacle region. The motile organs are cirri and cilia, all inserted in the constriction. There are two sets of cirri and one of cilia; the latter stand out radially from the girdle and are usually in motion. The cirri of one set, the anterior, extend forward about twice the length of the anterior half; those of the posterior set closely engirdle the lower half, reaching not quite to the posterior extremity. These are somewhat hyaline and are closely approximated, giving the impression of a tight-fitting crenulate casing about the lower half. The cirri are sharply pointed, much broader at the base, and the two sets are so placed that, looked at from above, they have the appearance of a twisted cord. (Fig. 31 b.) Movement erratic; sometimes the animal swims steadily forward with mouth in front; again it shoots across the field of the microscope, either backward or forward or sideways, through the action of its powerful cirri. It is often quiet, usually mouth downward, and is held in place by adhesion of the tentacles. In this position it looks strikingly like a heliozoön.

Length 35µ; greatest width 30µ. Not uncommon.

The chief features by which this species is distinguished from the frequently described M. pulex of Europe are the number of anterior cirri and the ring of true cilia in place of the central girdle of cirri. The European form is described with four anterior bristles; the present form has from 28 to 32. The radial cilia differ decidedly from the more powerful cirri and they are not in one plane, so that counting is difficult; they are not closely set. The presence of tentacles makes these forms of especial theoretical interest, especially in the light of the origin of Suctoria.

Genus TIARINA R. S. Bergh '79.

Body subcylindrical, pointed posteriorly, two and one-half times as long as broad; encased in covering composed of separate pieces arranged in five girdles. The pieces bear processes which rest against neighboring pieces of the girdle. Mouth large, anus terminal near contractile vacuole. The macronucleus is simple and round. Salt water.

Tiarina fusus (Cl. & Lach.) emend R. S. Bergh.

Synonyms: Coleps fusus Cl. & Lach. '58; Daday '86; Möbius '88, Lauterborn '94; Shevyakov '86.

This form, which resembles Coleps rather closely, was placed as a separate genus by R. S. Bergh. The skeletal parts consist of five zones of needles composed of an organized substance and embedded in the cortical plasm, the last zone coming to a point at the posterior end. The needles have lateral processes, which give a latticed appearance to the casing. The cilia are long, with a specialized crown of still longer ones at the oral end; they arise outside of the skeletal elements and do not pass between them, as in Coleps.

Genus LOXOPHYLLUM Dujardin '41.